ca2+ influx and protein scaffolding via trpc3 Search Results


k ca 2 3  (Alomone Labs)
94
Alomone Labs k ca 2 3
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
K Ca 2 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k ca 2 3/product/Alomone Labs
Average 94 stars, based on 1 article reviews
k ca 2 3 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
ca2+ influx and protein scaffolding via trpc3  (Putney Inc)
90
Putney Inc ca2+ influx and protein scaffolding via trpc3
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Ca2+ Influx And Protein Scaffolding Via Trpc3, supplied by Putney Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca2+ influx and protein scaffolding via trpc3/product/Putney Inc
Average 90 stars, based on 1 article reviews
ca2+ influx and protein scaffolding via trpc3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tg-evoked ca2+ response  (Putney Inc)
90
Putney Inc tg-evoked ca2+ response
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Tg Evoked Ca2+ Response, supplied by Putney Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tg-evoked ca2+ response/product/Putney Inc
Average 90 stars, based on 1 article reviews
tg-evoked ca2+ response - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
ca2 influx  (Putney Inc)
90
Putney Inc ca2 influx
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Ca2 Influx, supplied by Putney Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca2 influx/product/Putney Inc
Average 90 stars, based on 1 article reviews
ca2 influx - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
ca 2  (Proteintech)
93
Proteintech ca 2
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Ca 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca 2/product/Proteintech
Average 93 stars, based on 1 article reviews
ca 2 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
l-type ca2+ channel  (Thermo Fisher)
90
Thermo Fisher l-type ca2+ channel
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
L Type Ca2+ Channel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l-type ca2+ channel/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
l-type ca2+ channel - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
pmaxgfp  (Amaxa)
90
Amaxa pmaxgfp
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Pmaxgfp, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmaxgfp/product/Amaxa
Average 90 stars, based on 1 article reviews
pmaxgfp - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
fugene® hd  (Promega)
90
Promega fugene® hd
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Fugene® Hd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fugene® hd/product/Promega
Average 90 stars, based on 1 article reviews
fugene® hd - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
antibodies against dysferlin  (Novocastra)
90
Novocastra antibodies against dysferlin
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Antibodies Against Dysferlin, supplied by Novocastra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against dysferlin/product/Novocastra
Average 90 stars, based on 1 article reviews
antibodies against dysferlin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti ncx1  (Alomone Labs)
93
Alomone Labs anti ncx1
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Anti Ncx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ncx1/product/Alomone Labs
Average 93 stars, based on 1 article reviews
anti ncx1 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
gapdh  (Millipore)
90
Millipore gapdh
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh/product/Millipore
Average 90 stars, based on 1 article reviews
gapdh - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
monoclonal mouse anti-ncx1.3  (Swant)
90
Swant monoclonal mouse anti-ncx1.3
TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.
Monoclonal Mouse Anti Ncx1.3, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal mouse anti-ncx1.3/product/Swant
Average 90 stars, based on 1 article reviews
monoclonal mouse anti-ncx1.3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.

Journal: Scientific Reports

Article Title: Impairment of Coronary Endothelial Function by Hypoxia-Reoxygenation Involves TRPC3 Inhibition-mediated K Ca Channel Dysfunction: Implication in Ischemia-Reperfusion Injury

doi: 10.1038/s41598-017-06247-3

Figure Lengend Snippet: TRPC3 channels do not physically interact with IK Ca and SK Ca channels in PCAECs. The cell lysates were incubated with anti-TRPC3 antibody, and the immunoprecipitates were subjected to immunoblot by anti-K Ca 3.1 and anti-K Ca 2.3 antibodies respectively ( a and b ). The immunoprecipitation and immunoblot experiments were further performed in reversed order ( c and d ). Lysate supernatant (20 μg of protein) was used as input control. Mouse IgG was used as a negative control. Full-length western blot images are presented in Supplementary Figure . Experiments in each order were performed for four times with using cell isolates from different hearts.

Article Snippet: Extracted protein (1000 μg) was incubated overnight with 10 μg of antibody against TRPC3 (Alomone Labs), K Ca 2.3 (Alomone Labs), or K Ca 3.1 (Alomone Labs) at 4 °C on a rocking platform.

Techniques: Incubation, Western Blot, Immunoprecipitation, Negative Control